SHP-1 is required for TSR-mediated inhibition of VEGF-induced MVEC migration. (A) MVEC transfected with specific siRNA as in Figure 2 were allowed to migrate toward VEGF for 15 hours through an 8.0-μm membrane in a Transwell insert in the presence or absence of 10 nmol/L TSR. Within each group, siRNA transfected MVEC not exposed to VEGF or TSR served as controls. The number of migrated cells was counted and the result expressed as percent of cells migrated compared with control (n = 8). (B) Migration assays as in panel A were performed with MVEC treated with Src inhibitor (PP2) or Syk inhibitor (Piceatannol) (n = 6). PP3 served as a negative control for PP2.