Genetic deletion of cd36 increases VEGFR2 phosphorylation and decreases SHP-1 association with VEGFR2 after in vivo infusion of VEGF. (A) Lysates of lung tissue removed from cd36 null or wild-type mice were analyzed by western blot with anti-VEGFR2, anti-CD36, or antitubulin (top) or by immunoprecipitation with anti-VEGFR2 followed by western blot (bottom). (B) Lung tissue from wild-type or cd36 null mice was harvested 5 minutes after injection of 1 μg VEGF via the jugular vein. The top panel shows VEGFR2 Tyr1175 phosphorylation detected by western blot analysis of tissue lysates. Blots were reprobed with antibodies to total VEGFR2, CD36, and tubulin. The bottom panel shows western blot analysis of anti-VEGFR2 immunoprecipitates of the tissue lysates probed with antibodies to SHP-1 and VEGFR2. Blots are representative of 3 experiments. (C) VEGFR2 phosphorylation levels in panel B were expressed as the ratio of phosphorylated VEGFR2 to total VEGFR2.