Poor recognition of shorter peptides by the SB27 TCR. T2-HLA B*3508 cells (6 × 10⁴) were pulsed with the indicated 9mer (A-B) or 13mer (C-D) peptides at the concentrations depicted for 2 hours at 37°C. Subsequently, 3 × 10⁴ SB27 CD8⁺ T cells were added and incubated overnight. The supernatant was then harvested and assayed for MIP1β by ELISA. Error bars represent SDs.