Assessment of the LIC content of sorted ALL subpopulations. B-ALL cells from 8 patients (patients 2, 6, 16, 17, 20, 22, 23, and 28) were sorted for expression of CD34 and CD19 (A) and cells from 7 T-ALL patients (patients 29-32 and 34-36) were sorted for expression of CD34 and CD7 (B). Unsorted cells and sorted subpopulations were inoculated into NSG mice to evaluate the LIC content. Two to 4 mice were injected per cell dose. Each patient is represented by a specific symbol and each symbol depicts the leukemia engraftment detected in the BM of an individual mouse.