Intracellular localization of αDEC-205 in LCLs and mDCs. (A) LCLs and mDCs were incubated with αDEC-205, washed and incubated for 0 or 30 minutes at 37°C. Cells were then permeabilized, fixed, and stained with antibodies to transferrin receptor (TR) or lysosomal-associated membrane protein 2 (LAMP-2), αDEC-205 (DEC), and DAPI. Scale bars represent 5 μm. Representative cells from 1 experiment of 3 are shown. (B) Quantitative analysis for colocalization of αDEC-205 with TR and LAMP-2 in LCLs and mDCs loaded with αDEC-205 and incubated for 30 minutes at 37°C. Fifteen to 20 cells were analyzed from 1 independent experiment, data represent means from 50 to 60 cells from 3 experiments. Error bars indicate SD.