YOD1-C160S expressing BMDCs enhance antigen cross-presentation. (A) A schematic of antigen cross-presentation experiments is shown. Control and YOD1-C160S expressing BMDCs were either pulsed with Ova or were fed apoptotic MHV-68–infected MEFs and cocultured with OT-I cells or ORF8 TN cells, respectively. (B-C) FACS plots (B) and bar diagram (C) show expression of CD69 by OT-I cells measured after 18 hours of cocultures with BMDCs that were pulsed with indicated concentrations of ovalbumin. (D-E) Proliferation (D) and IL-2 production (E) by OT-I cells was measured after 60 hours of coculture with Ova-pulsed control and YOD1-C160S BMDCs at indicated concentrations of Ova. (F-G) Proliferation (F) and IL-2 production (G) by ORF8 TN cells was measured after 72 hours of coculture with control and YOD1-C160S BMDCs, which were fed with MHV-68–infected apoptotic MEFs. All experiments were repeated at least 3 times and data are represented as mean ± SEM.