Cell-surface marker expression in FOXP3-deficient Th1 and Th17 cells. Th1 and Th17 cells transduced with siFOXP3 or control siLuc and purified based on ΔLNGFR expression were restimulated with anti-CD3/anti-CD28–coated beads (1 bead: 32 cells) in the presence of IL-2. At the indicated days after activation, cell-surface marker expression was determined by flow cytometry. Analysis was conducted on ΔLNGFR⁺ cells. The top panels of A-D show mean fluorescence intensities (MFIs) of one representative experiment. The bottom panels of A-D show the average fold change in MFI of siFOXP3 relative to siLuc (MFI siFOXP3/ MFI siLuc). (A) Th17 CCR4 (n = 3-4). (B) Th17 CCR6 (n = 2-3). (C) Th17 CTLA-4 (n = 2-3). (D) Th1 CTLA-4 (n = 2-3). Error bars represent SEM. *P < .05; **P < .01.