The induction of tolerogenicity in mDCs by MSCs was dependent on TIEG1. The mDCs infected with TIEG1 shRNA (h) lentivirus or control shRNA lentivirus were incubated with MSCs for 3 days and sorted using immunomagnetic beads. The sorted DCs were then cocultured with CFSE-labeled allogeneic CD4⁺ T cells for 3 days, and analyzed for T-cell proliferation. Each bar represents the division index of lymphocyte proliferation. LYM, CFSE-labeled CD4⁺ T lymphocytes. mDCs + LYM, labeled T cells cocultured with mDCs. MSC-DCs (TIEG1 shRNA) + LYM, labeled T cells cocultured with lentivirus-infected mDCs after incubation with MSCs. MSC-DCs + LYM, labeled T cells cocultured with mDCs after incubation with MSCs. MSC-DCs (control shRNA) + LYM, labeled T cells cocultured with control lentivirus-infected mDCs after incubation with MSCs. Data represent median with interquartile range from 3 independent experiments. Differences between 2 groups were compared using the Mann-Whitney U test. *P < .05; **P < .01.