Figure 3
Figure 3. Elevated expression of Gata3 in E2A−/− DN2 cells in vivo and in vitro. (A) qPCR analysis shows Gata3 mRNA in ETPs, DN2, DN3, and DN4 cells from E2A+/+ (light blue), E2A+/− (medium blue), and E2A−/− (dark blue) mice. One of 4 representative experiments is shown with the SE from triplicate reactions. (B) qPCR analysis for Gata3 mRNA in E2A+/+ (light shade) or E2A−/− (dark shade) FL MPPs cultured on OP9 (green) or OP9-DL1 (blue) with Flt3L and IL-7 for 4, 6, or 8 days. One of 2 experiments is shown with error bars from triplicate measurements. (C) FACS analysis shows expression of GATA3 in ETPs and DN2, DN3, and DN4 cells from E2A+/+ (blue) and E2A−/− (red) mice or (D) DN1, DN2, DN3, or NK1.1+ cells from in E2A+/+ (blue), E2A+/− (green), and E2A−/− (red) in FL MPPs after 10 days of culture. Negative control staining is shown in gray. One of at least 3 experiments is shown.

Elevated expression of Gata3 in E2A−/− DN2 cells in vivo and in vitro. (A) qPCR analysis shows Gata3 mRNA in ETPs, DN2, DN3, and DN4 cells from E2A+/+ (light blue), E2A+/− (medium blue), and E2A−/− (dark blue) mice. One of 4 representative experiments is shown with the SE from triplicate reactions. (B) qPCR analysis for Gata3 mRNA in E2A+/+ (light shade) or E2A−/− (dark shade) FL MPPs cultured on OP9 (green) or OP9-DL1 (blue) with Flt3L and IL-7 for 4, 6, or 8 days. One of 2 experiments is shown with error bars from triplicate measurements. (C) FACS analysis shows expression of GATA3 in ETPs and DN2, DN3, and DN4 cells from E2A+/+ (blue) and E2A−/− (red) mice or (D) DN1, DN2, DN3, or NK1.1+ cells from in E2A+/+ (blue), E2A+/− (green), and E2A−/− (red) in FL MPPs after 10 days of culture. Negative control staining is shown in gray. One of at least 3 experiments is shown.

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