Heterozygous deletion of Gata3 restores DN3-like cell development from E2A^−/− FL MPPs. (A) FACS analysis for CD44 and CD25 on the progeny of Lin⁻ FL cells from control (including WT, Cre⁺, or Gata3^+/fl), E2A^−/−, or E2A^−/−Gata3^+/Δ embyros after culture on OP9-DL1 for 12 days. Data are representative from at least 3 experiments. (B) Average + SE derived from 6 mice of each genotype. (C) qPCR analysis for Gata3 or (D) Notch1 and Rag1 mRNA isolated from sorted DN1-like (white), DN2-like (gray), or DN3-like (black) cells isolated from the cultures shown in panel A. Expression is shown relative to WT DN1 cells. NA indicates not available. Data are from 1 of 2 similar experiments.