Cell intrinsic functions of DOCK8 regulate NKT cell selection and the number of NKT cells in the thymus and liver. (A) Mixed bone marrow chimeras generated by reconstituting irradiated CD45.1 allotype WT mice with BM derived from 80% WT CD45.2 or 80% DOCK8^cpm/cpm (CPM) CD45.2 mice mixed with 20% CD45.1 WT BM. Bars show individual chimeric mice and reconstitution levels expressed as a percentage of allotype-specific αGalCer-tetramer⁺ NKT cells. Data are representative of 3 independent experiments using mixed chimeras. (B) Relative expression levels of the maturation marker NK1.1 on NKT cells in WT and DOCK8^cpm/cpm (CPM) NKT cells from the liver and spleen representative of 4 biological replicates.