Downregulation of Lyl1 during differentiation of wild-type and Lmo2-transgenic thymocytes. (A) Expression of Lmo2, Lyl1, and their target genes during normal T-cell differentiation. Data from the Immgen Database³⁷  were used to determine the expression levels of the indicated genes during normal αβ T-cell differentiation. ETP, early thymocyte precursor; ISP, CD8⁺ intermediate single-positive cell. (B) Downregulation of Lmo2 target genes during T-cell differentiation in Lmo2-transgenic mice. DN and DP thymocytes were sorted from 8-week-old mice of the indicated genotypes and used to generate cDNA, which was amplified by real-time PCR using primers specific for the indicated genes. Data are represented as mean +SD of triplicate values and are representative of 3 separate experiments.