Figure 6
The Lmo2-associated gene expression program is restricted to CSCs. (A) Tumor samples derived from Lmo2-transgenic mice were fractionated into the indicated populations, and 3 × 105 cells of each population were injected into irradiated congenic (Ly5.1) recipients. The survival curves of mice receiving the indicated tumor cell populations are shown on the right. (B) FACS profile of secondary tumors from mice receiving the indicated primary tumor populations from mouse 575. (C) DN and DP cells were sorted from 2-month-old wild-type (wt) or Lmo2-transgenic mice, or from Lmo2-transgenic tumor 575 (as in A), and the expression levels of the indicated genes were determined by real-time PCR.
