Expansion of CD8α⁺ DCs after short-term bone marrow reconstitution. Analyses of purified CD11c⁺ DCs from bone marrow chimeric Id2^gfp/gfp (Ly5.2⁺)→Ly5.1⁺, Id2^gfp/gfpBatf3^−/− (Ly5.2⁺)→Ly5.1⁺ mice 21 days after reconstitution. DCs were purified from (A) spleen (top panels) and peripheral LNs (bottom panels) then stained for various surface molecules and analyzed by flow cytometry. Numbers indicate the percentage of cells in each DC subset gated on live (PI⁻) donor (Ly5.1⁻ or Ly5.2⁻ cells) CD11c⁺CD45RA⁻ DCs. (C) Proportion and total number of CD8α⁺ DCs recovered from spleens of chimeric mice as in panel A. Data are representative of at least 3 separate experiments with similar results (n = 6 mice per group). (D) Analysis of splenocytes (left panel) and DCs (right panel) purified from spleens of Ly5.1⁺:Batf3^−/− (Ly5.2⁺)→Ly5.1/5.2⁺ F1 mixed bone marrow chimeras and analyzed by flow cytometry. Numbers indicate the percentage of cells in each DC subset gated on live (PI⁻) donor (Ly5.1⁺ or Ly5.2⁺ cells) bone marrow. Data are representative of at least 2 separate experiments with similar results (n = 5-6 mice per group).