Figure 5
Figure 5. Loss of Bif-1 suppresses mitophagy. Bif-1+/+ and Bif-1−/− mouse embryonic fibroblasts were transduced with lentiviruses encoding myc-Parkin or mCherry-Parkin and selected with puromycin for 5 days. (A) The resultant stable clones were prestained with MitoTracker CMTMRos, treated with 30μM CCCP or control DMSO for the indicated periods of time, and analyzed by fluorescent microscopy. Magnified images are shown in the insets. (B) Cells were treated with 30μM CCCP for 0, 6, and 24 hours and immunostained for Tom20 and Hsp60. Data shown are representative of the 24-hour time point; images at the 0- and 6-hour time points are shown in supplemental Figure 2. Arrows represent Hsp60+ and Tom20− structures. (C-D) Cells treated with 30μM CCCP for the indicated periods of time were subjected to immunoblot analysis using the indicated antibodies. The scale bars in panels A and B represent 10 μm.

Loss of Bif-1 suppresses mitophagy. Bif-1+/+ and Bif-1−/− mouse embryonic fibroblasts were transduced with lentiviruses encoding myc-Parkin or mCherry-Parkin and selected with puromycin for 5 days. (A) The resultant stable clones were prestained with MitoTracker CMTMRos, treated with 30μM CCCP or control DMSO for the indicated periods of time, and analyzed by fluorescent microscopy. Magnified images are shown in the insets. (B) Cells were treated with 30μM CCCP for 0, 6, and 24 hours and immunostained for Tom20 and Hsp60. Data shown are representative of the 24-hour time point; images at the 0- and 6-hour time points are shown in supplemental Figure 2. Arrows represent Hsp60+ and Tom20− structures. (C-D) Cells treated with 30μM CCCP for the indicated periods of time were subjected to immunoblot analysis using the indicated antibodies. The scale bars in panels A and B represent 10 μm.

Close Modal
This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal