CD244, CD160, and PD1 are preferentially expressed on CD3⁺CD8⁺CCR7⁻ effector T cells which are increased in CLL. (A) CLL patients had an increased proportion (A) and increased numbers (B) of CD3⁺CD8⁺CCR7⁻ effector T cells. The relative expression of CD160, CD244, and PD1 was analyzed on CD8⁺ lymphocyte subsets from CLL patients as defined by coexpression of CCR7 and CD45RA. Quadrant gates were used to compare the MFI of CD160, CD244, and PD1 on CCR7⁺CD45RA⁺ naive cells, CCR7⁺CD45RA⁻ T_CM cells, CCR7⁻CD45RA⁻ T_EM cells, and CCR7⁻CD45RA⁺ T_EMRA cells. Expression of CD244 (C) and CD160 (D) was increased on T_EM cells, and was highest on T_EMRA cells. (E) Expression of CD244 was virtually specific for a CCR7⁻ phenotype. (F) In contrast, expression of PD1 was highest on T_EM cells.