Thin layer chromatography staining of total A_pae glycolipids. (A) Nomenclature and structures of selected glycolipids discussed in this paper. (B) Helix pomatia lectin staining of the A_pae total glycolipid samples and blood group A₁, A_2, O, and B controls plus Fs glycolipid control. TLC migratory positions of known blood group glycolipids are identified in the margin. (C) Staining with MAb anti-Fs. (D) Staining with a representative broadly reactive MAb anti-A (clone 11H5). (E) Unidentified bands recognized by staining with MAb anti-A (003). (F) Staining with Lewis (anti-Le^a, anti-Le^b) and secretory A antigen glycolipid profiles (anti-A type 1, anti-ALe^b). In all panels A₁, A₂, O, and B control samples are total erythrocyte glycolipids whereas Fs is a purified glycolipid fraction. Lewis phenotypes are expressed as red cell phenotypes [where low level Le^a glycolipid is expected in the Le(a−b+) phenotype]. Because of minor migration differences between assays, direct comparison of absolute band positions should be viewed with caution. Black lines indicate where migration controls and unloaded lanes have been removed from the image. All lanes are from the same TLC plate and have not been repositioned.