The consistent macrophage signature predicts in situ macrophage-specific expression in human tissues. The gene signature of resting macrophages detected by proteomics and microarray ranking can be detected in lung-resident tissue macrophages. Shown are selected immunohistochemical stainings from human lung tissue using antibodies against CD68, Galectin-3 (LGALS3), Peroxiredoxin (PPRDX1), S100A4, Annexin A1 (ANXA1), ITGB2 (Integrin β 2, CD18), Niemann Pick type C2 (NPC2), cathepsins (CTSB, CTSD, CTSH, CTSZ), ATPase, H⁺ transporting, lysosomal V1 subunit B2 (ATP6V1B2), Prosaposin (PSAP), fatty acid binding protein 5 (FABP5), actin β (ACTB), ribosomal protein S20 (RPS20), ribosomal protein L27 (RPL27), and the mitochondrial protein ubiquinol-cytochrome c reductase core protein (UQCRC1). Data were generated with the Human Protein Atlas and staining was selected only if the antibodies had supportive scores for immunohistochemistry.