Neddylation inhibition in BMDC mitigates release of non-TLR4–stimulated cytokines in vitro. ELISA quantification of TNF-α release in Pam3CSK4 (300 ng/mL) stimulated (A), PGN (5 μg/mL) stimulated (B), and CD40L (1 μg/mL) stimulated (C) BMDC in the presence or absence of vehicle, MLN4924, or dexamethasone at the indicated doses. (D) Comparison of TNF-α release via ELISA of cells treated with vehicle, MLN4924, dexamethasone, or bortezomib followed by concurrent stimulation with LPS (0.5 µg/mL). Cells receiving treatment were preincubated with vehicle, MLN4924, dexamethasone, or bortezomib for 2 hours followed by concurrent stimulation for 4 hours. One representative experiment of 3 is shown in cells treated in triplicate. *P < .05; **P < .01; ***P < .0001.