Neddylation inhibition mitigates allogeneic T-cell proliferation. BALB/C T cells were cultured with C57BL/6 irradiated (30 Gy) BMDC at 40:1 and 100:1 ratios in the presence or absence of MLN4924 for 72 hours (A) and 96 hours (B). Where indicated, BMDC were preincubated in the presence or absence of vehicle or MLN4924 at specified dosage for 6 hours. One representative experiment of 3 is shown of groups treated in triplicate. *P < .05; **P < .01; ***P < .0001.