Human hematolymphoid reconstitution at 16 weeks after transplantation. (A) In the BM, BRGS mice showed sustained multilineage engraftment of human hematopoiesis at a level comparable to that in NOD-RG mice (●: BRGS female; ▴: NOD-RG female). (B) In the thymus, CD3⁺ T cells were developed and their frequencies were comparable in BRGS and NOD-RG mice. On FACS analysis, cells were differentiated into CD4⁺ and CD8⁺ single-positive T cells expressing the surface TCR-αβ chain. (C) In the spleen, CD10⁻CD19⁺CD20⁺ mature B cells expressing surface Ig light chain λ or κ chain were present.