Figure 6
Figure 6. Reactivation of viral gene expression from HTLV-1 latently infected ATLL cells. (A) Taqman quantification of tax/rex RNA copy number per million GAPDH in MT-1, TL-Om1, and 8 additional uncultured ATLL samples. Standard deviations were calculated from 4 independent experiments. (B) RT-PCR detection of p30 RNA expression in TL-Om1 and MT-1 cells using LTR2 and TF4 for 43 cycles. (C) Western blot analyses showing knockdown (KD) of PA28γ expression in MT-1KD and TL-Om1KD cell lines. (D) Taqman quantification of tax/rex RNA copy number per million GAPDH in MT-1, MT-1KD, TL-Om1, and TL-Om1KD cells. Standard deviations were calculated from 2 independent experiments. Agarose gel amplification is shown underneath. (E) Intracellular staining for detection of Tax expression by FACS in MT-1 parental cell line and MT-1KD cells using Tax monoclonal antibody Tab172. (F) Taqman quantification of tax/rex RNA copy number per million GAPDH in TL-Om1 and TL-Om1KD treated with decitabine (1μM) for 72 hours. Standard deviations were calculated from 2 independent experiments. (G) Intracellular staining for detection of Tax expression by FACS in TL-Om1 parental cell line and in TL-Om1KD cells treated with decitabine using Tax monoclonal antibody Tab172.

Reactivation of viral gene expression from HTLV-1 latently infected ATLL cells. (A) Taqman quantification of tax/rex RNA copy number per million GAPDH in MT-1, TL-Om1, and 8 additional uncultured ATLL samples. Standard deviations were calculated from 4 independent experiments. (B) RT-PCR detection of p30 RNA expression in TL-Om1 and MT-1 cells using LTR2 and TF4 for 43 cycles. (C) Western blot analyses showing knockdown (KD) of PA28γ expression in MT-1KD and TL-Om1KD cell lines. (D) Taqman quantification of tax/rex RNA copy number per million GAPDH in MT-1, MT-1KD, TL-Om1, and TL-Om1KD cells. Standard deviations were calculated from 2 independent experiments. Agarose gel amplification is shown underneath. (E) Intracellular staining for detection of Tax expression by FACS in MT-1 parental cell line and MT-1KD cells using Tax monoclonal antibody Tab172. (F) Taqman quantification of tax/rex RNA copy number per million GAPDH in TL-Om1 and TL-Om1KD treated with decitabine (1μM) for 72 hours. Standard deviations were calculated from 2 independent experiments. (G) Intracellular staining for detection of Tax expression by FACS in TL-Om1 parental cell line and in TL-Om1KD cells treated with decitabine using Tax monoclonal antibody Tab172.

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