Differential gene expression between WT and Mysm1^−/− LSK cells. (A) Mouse RT² Profiler PCR array was performed with sorted LSK cells from WT and Mysm1^−/− mice. Solid line indicates no change in gene expression of 1. Dotted lines indicate fold-change (2^(−DCt)) of greater than or equal to 2. (B) Real-time PCR analysis of Notch signaling molecules and important HSC transcriptional factors in WT and Mysm1^−/− LSK cells. (C) Sorted LSK cells from WT and Mysm1^−/− mice were infected with lentivirus encoding GFP (pCDH-GFP) or Mysm1 (pCDH-Mysm1). Gfi1 expression was assessed 72 hours later by real-time PCR. The Ct values are normalized to glyceraldehydes-3-phosphate dehydrogenase and are presented relative to WT (value set as 1). (B-C) Data are representative of 3 independent experiments. *P < .05.