Mysm1 orchestrates histone modifications at the Gfi1 locus. ChIP assays were performed in WT and Mysm1^−/− Lin⁻ cells using (A) anti-IgG, (B) anti-ubH2A, (C) anti-Ring1b, (D) anti-Bmi1, (E) anti-H3K4me3, (F) anti-H3K27me3, and (G) anti-RNA pol II antibodies. The precipitated DNA was analyzed by real-time PCR using primers along the Gfi1 promoter region (primer set C in Figure 5A). Primer amplifying the coding region (fourth exon) of Gfi1 was used as a negative control. The relative amount of immunoprecipitated DNA was presented as a percentage of input DNA. Data are representative of three independent experiments. *P < .05.