Treatment with 12B9m led to more effective red cell hemoglobinization and aided in reticulocyte production. (A) Experimental scheme detailing administration time of intraperitoneal BA (3 × 108 particles per mouse), intravenous Ab at doses stated, and intravenous ESA (300 µg/kg Epoetin alfa) in Hep1 mice. Hb was monitored on day 7 and used to assign mice to groups as in Figure 2. Hb (B), RBC number (C), and mean corpuscular Hb (MCH) (D) were measured from 1 study on day 14 (n = 4-5 mice per group). Reticulocyte mean corpuscular Hb (CHr) (E), reticulocyte number (F), and reticulocyte mean corpuscular volume (MCV) (G) were measured from an analogous study on day 12 (n = 4-5 mice per group). Statistical comparisons against control Ab group are shown (1-way ANOVA with Dunnett’s post hoc test). *P < .05; **P < .01; ***P < .001. All results are shown as mean ± SEM.
