Treatment with 12B9m did not impact inflammatory cytokine or erythropoietin production in mice with AI. Hep1 mice were treated with either intraperitoneal saline or BA (3 × 108 particles per mouse) and intravenous 12B9m or control Ab (5 mg per mouse). Changes in serum concentration of IL-6 (A), IL-1β (B), TNF-α (C), and GM-CSF (D) were measured 6 hours after treatment (n = 3 mice per group). Statistical comparisons against BA + control Ab group are shown (1-way ANOVA with Dunnett’s post hoc test). ***P < .001. (E) In a parallel study, endogenous mouse erythropoietin (mEPO) concentration was measured at days 0, 7, 14, 21, and 28 (n = 4-6 mice per group per time point; 1 value in saline treatment group at day 14 was excluded because the value was >10-fold higher than all other values for saline treatment). Statistical comparisons against BA + control Ab group over time were conducted (2-way ANOVA with Bonferroni post hoc test; no significance). (F) Repeat study examining mEPO at day 14 to confirm results (n = 2-5 mice per group per time point). Statistical comparisons against BA + control Ab group were conducted (1-way ANOVA with Dunnett’s post hoc test; no significance). Results are shown as mean ± SEM. GM-CSF, granulocyte macrophage–colony-stimulating factor; TNF-α, tumor necrosis factor.
