Quantification of wt-platelet or V1316M-platelet recovery in liver and spleen of wt-expressing and p.V1316M-mVWF–expressing mice. Liver (A-B) and spleen (D-E) cryosections were prepared as described in the legend of Figure 5 and scanned with a NanoZoomer. Images comprising whole sections were generated via virtual microscopy technology using NDPview software. Quantification (C,F) was performed using ImageJ software and is expressed as the percentage of surface covered by fluorescent spots (corresponding to fluorescent platelets) per field. Data represent mean ± SEM. The number of fields analyzed is indicated. **P = .0098; ***P = .0002. Representative images for cryosections of liver (A-B) and spleen (D-E) obtained from wt-mVWF–expressing mice (A-D) or p.V1316M-mVWF–expressing mice (B-E) are shown. Arrows point to fluorescent spots.