ERG ChIP-Seq in TgERG mice. (A) Validation of ChIP quality by quantitative RT-PCR of the Hhex +1-Kb enhancer-fold enrichment compared with the IgG control in 2 TgERG leukemias. (B) Density plots visualizing ERG binding in 3 known hematopoietic target gene loci, Hhex, Nfe2, and Runx-1. (C) Venn diagram intersecting ERG peaks in the 2 Tg leukemias shows marked similarity between 2 samples. (D) Pie chart showing ERG distribution across genomic regions using the designated high-confidence peaks. (E) De novo motif discovery performed on ERG high-confidence peaks identified ETS and GATA motifs as highly enriched. (F) Biological function of ERG-bound genes as identified by the Genomic Regions Enrichment of Annotations Tool. A table summarizing the most enriched mouse phenotypes is presented and phenotypes related to mega-erythro lineage are highlighted in yellow.