Integration of ChIP-Seq and expression data reveals transcriptional programs regulated by ERG in Tg leukemia. (A) Heat map depicting gene expression of ERG-bound genes in TgERG leukemia compared with the WT lin^– controls. (B) GSEA using upregulated transcription factors (TFs) in Tg-ERG leukemia shows significant enrichment in human AML progenitors (LPC) compared with normal hematopoietic progenitors (HPC²⁰ ). (C) Validation of Gata2 and Hey2 upregulation in TgERG leukemia compared with WT bone marrow, WT lin^– bone marrow, and WT MEPs. Quantitative RT-PCR results normalized to β-actin are shown (mean ± SEM, n = 3 for WT, WT lin^–, and WT MEPs; n = 5 for TgERG leukemia).