Figure 7
![Figure 7. Specificity of the Blockmir. (A) Western blot analyses of VE-cadherin, SEMA6A from HUVECs transfected with the LNA27, CD5-2, or control; α-tubulin was used as a loading control. (B) Quantitative analysis as described in (A): control (white bar), LNA27 (hatched bar), and CD5-2 (black bar); mean ± SEM, *P < .05 (n = 3-5). (C) VE-cadherin and (D) PPARγ reporter activity from cells co-transfected with mimic control (Vector[−]) or miR-27a-mimic only (Vector), or miR-27a-mimic plus Blockmirs control or CD5-2. (C) Mean ± SD from 3 independent experiments with 3 replicate samples per experiment; analysis of variance *P ≤ .05. (D) *P < .05 from a representative experiment.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/122/16/10.1182_blood-2012-12-473017/4/2911f7.jpeg?Expires=1735829964&Signature=T2inSyfBecZJ7XQ0AK3vdyCYLs7PuN5EQVpdV9zy674ddrCZf1wFa3S3mwOQrIE72snRIq27mI22KcYp2R7IQTB4O9-i58bIKIb4AscOmViOjtPstC-sX5KH0ILVux2g7I7oahpbrQZSv6y2p7RpItf64MnkliQ4dFL6ORw9NTHSc913lsc8yiIzuDzOx8kifSyH3FLks8xn7iyOo18QAevUmJva448Abfv7kNhm2tl2I5Dfg0sor7cMIRBZAR3dqNvmhvxmKPMh~prXaFV5E5QND-KtLvVZ5vDP79o5NXuQgHBoNOv9R3BO5tEp1OrtYeGSgaV0DqzaYKITeaurCg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Specificity of the Blockmir. (A) Western blot analyses of VE-cadherin, SEMA6A from HUVECs transfected with the LNA27, CD5-2, or control; α-tubulin was used as a loading control. (B) Quantitative analysis as described in (A): control (white bar), LNA27 (hatched bar), and CD5-2 (black bar); mean ± SEM, *P < .05 (n = 3-5). (C) VE-cadherin and (D) PPARγ reporter activity from cells co-transfected with mimic control (Vector[−]) or miR-27a-mimic only (Vector), or miR-27a-mimic plus Blockmirs control or CD5-2. (C) Mean ± SD from 3 independent experiments with 3 replicate samples per experiment; analysis of variance *P ≤ .05. (D) *P < .05 from a representative experiment.
