Effect of acute Rictor deletion on B lymphoid cells.ROSA26-Cre-ER^T2Rictor^fl/fl mice were treated with tamoxifen as described in “Methods.” (A) PCR was performed to determine Rictor excision using peripheral B cells of mice of the indicated genotypes 2 weeks after starting tamoxifen injection as in Figure 1A. (B) Shown are mean (± SEM) numbers of B lineage cells in the spleen and BM of Cre-ER^T2Rictor^fl/fl (cKO) or control (WT) mice 4 weeks after starting tamoxifen treatment (6 WT vs 6 cKO; 2 separate replicate experiments, each of 3 vs 3 mice). (C) Representative FACS profiles with frequencies of the indicated subsets in the viable lymphoid gates of the spleen or further gates as indicated in the above panels. (D) Decreased recirculating mature B cells in the BM of Rictor-deficient mice. Shown are representative FACS profiles with frequencies of IgD^hi CD23^hi cells in the B220⁺ gates (left) and mean (± SEM) numbers of the population in the BM of Cre-ER^T2Rictor^fl/fl (cKO) or control (WT) mice 4 weeks after tamoxifen treatment (right): n = 6 vs 6.