The density of H3K27 acetylation and size of the histone-free region surrounding a peak increases with the number of overlapping TFs at that peak. (A) A read density heat map of H3K27ac at 28 793 TF-bound regions. Although the vertical axis indicates the number of bound sites by different numbers of TFs, it is scaled to equal size. H3K27ac densities adjacent to a peak increase with the number of overlapping TFs at that peak. (B) Quantitative distribution of H3K27ac densities for regions bound by 1 or more TFs. In regions bound by only 1 TF, approximately 10% show no detectable H3K27ac and only ∼8 (log₂3) reads in up to 50%. In regions bound by all 7 TFs >90% of regions have more than ∼8 reads. (C) The average distances between H3K27ac peak densities flanking the TF peak maxima increases with the number of overlapping TFs within a peak. The corresponding increase in depth of the H3K27ac troughs reflects the increased H3K27ac read densities associated with a higher number of TFs.