American Society of Hematology

Figure 5

$Figure 5. Type 1 pre-HSCs are present in UC and VV. (A) Four populations sorted based on VE-cadherin and CD45 expression were coaggregated with OP9 cells and transplanted after 4 days of culture (with IL-3 + SCF + Flt3l). The level (%) of donor engraftment in PBL was assessed 14 weeks after transplantation (n = 7 independent experiments). HSC activity correlated with VE-cad+CD45+ and VE-cad+CD45– fractions. (B) Type 1 pre-HSCs (VE-cad+CD45–) from different embryonic tissues were coaggregated with OP9 cells and transplanted after 4 days of culture. Note that VV and UC contain type 1 pre-HSCs.$

Type 1 pre-HSCsare present in UC and VV. (A) Four populations sorted based on VE-cadherin and CD45 expression were coaggregated with OP9 cells and transplanted after 4 days of culture (with IL-3 + SCF + Flt3l). The level (%) of donor engraftment in PBL was assessed 14 weeks after transplantation (n = 7 independent experiments). HSC activity correlated with VE-cad⁺CD45⁺ and VE-cad⁺CD45^– fractions. (B) Type 1 pre-HSCs (VE-cad⁺CD45^–) from different embryonic tissues were coaggregated with OP9 cells and transplanted after 4 days of culture. Note that VV and UC contain type 1 pre-HSCs.

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