Reduction of kindlin-2 expression in WT ECs results in enhanced CD39 and CD73 expression and activities. WT and kindlin-2^+/− ECs were either untreated or treated with control or kindlin-2 siRNA. Untreated kindlin-2^+/− ECs served as control. (A) Western blot analysis of EC lysates using anti–kindlin-2 mAbs or Abs to actin as a loading control. (B) FACS analysis with anti-CD39 and anti-CD73 Abs reveal reduced CD39 and CD73 expression on kindlin-2 siRNA-treated WT ECs. The data are mean ± SEM of triplicate samples and are representative of 3 independent experiments. (C) CD39 (left panel) and CD73 (right panel) activities were analyzed on the EC surface as described in Figure 3C. The data are mean ± SEM of quadruple samples of 3 independent experiments. NT, nontreated.