Loss of Nfix leads to the downregulation of known regulators of the survival of HSPCs. (A) mCherry⁺ LSK cells were recovered via FACS from LSK cells transduced with either control- or Nfix-shRNA and cultured for 7 days ex vivo in serum-free medium supplemented with hematopoietic cytokines. The global transcriptional profiles of these cells were then acquired using Affymetrix gene chip technology. Data represent 1 experiment with 3 independent samples of each type. (B) Cells as in (A) were analyzed by qRT-PCR for mRNA expression. Data represent mean ± standard deviation from 2 independent experiments. P values were considered statistically significant when *P < .05, **P < .01, and ***P < .001.