Increased short-term myeloid cell output in NSG-3GS mouse BM and PB. Kinetics of myeloid cell reconstitution in the BM (A) and PB (B) of NSG (open circles) and NSG-3GS (closed circles) mice after their transplantation with 10⁴ lin⁻CD34⁺ CB cells. Data are the results of 12 mice per group pooled from 2 independent comparison experiments. (C) Total mouse BM cells per 2 femurs plus 2 tibias at the times indicated after irradiation of mice with 315 cGy ¹³⁷Cs but no transplanted cells (3-10 mice per time-point). (D) Linear relationship between the number of CD34⁺ CB cells transplanted and the extent of human myeloid cell chimerism produced in the BM of NSG and NSG-3GS mice 3 weeks after transplantation of 50 to 1.6 × 10⁴ CD34⁺ CB cells (3-33 mice per cell dose). (E) Linear relationship between the number of CD34⁺ CB cells transplanted and the absolute number of circulating human myeloid cells in NSG and NSG-3GS mice 3 weeks later (3-33 mice per cell dose). (F) Wright-Giemsa–stained human CD45⁺CD33/15⁺ cells in the blood of NSG-3GS mice 3 weeks after their transplantation with human CD34⁺ CB cells, representing > 90% of the sorted CD45⁺CD33/15⁺ cells analyzed. All values shown are the mean ± SEM. The dotted lines in panels D and E indicate the limit of detection of human CD45⁺CD33/15⁺ cells in BM and PB samples, respectively.