SH2B3 expression rescues deregulated cell signaling growth and proliferation in patient-derived SH2B3 mutant lymphoblastoid cells. (A) Western blot of patient-derived SH2B3-deficient lymphoblastoid cells reconstituted with empty vector (pLX301) or SH2B3 expressing lentiviruses (pLX301 SH2B3). (B) CFSE dilution assay analysis of cell cycle kinetics in SH2B3 mutant cells infected with empty vector or SH2B3 expressing lentiviruses (C,D). Differential cell growth of SH2B3 mutant cells infected with empty vector or SH2B3 expressing lentiviruses in standard (10% FBS) (C) and low-serum (1% FBS) (D) culture conditions. Experiments shown were performed twice, and quantitative assays were performed in triplicate.