Antiplatelet function of statins involves PECAM-1. Platelets derived from PECAM-1^+/+ and PECAM-1^−/− mice were treated with simvastatin (1-20 μM) for 5 minutes prior to stimulation with CRP-XL (1 μg mL⁻¹) and aggregation measured at 37°C under constant stirring conditions (A). Platelets derived from PECAM-1^+/+ and PECAM-1^−/− mice were treated with simvastatin (1-20 μM) for 5 minutes prior to stimulation with CRP-XL (1 μg mL⁻¹) and fibrinogen binding and P-selectin exposure were assessed by flow cytometry (B-C). Data are representative of 4 different experiments (n = 4); P values were calculated by nonparametric Mann-Whitney test (*P ≤ .05, **P ≤ .01) (A-C). Laser-induced thrombosis in PECAM-1^−/− mice in the presence and absence of fluvastatin (1-20 μM) or vehicle was investigated. Representative images of thrombi obtained from mice treated with or without fluvastatin (1 μM) at different time intervals after laser injury are shown. Arrows indicate the direction of blood flow (D). Median fluorescence intensity was measured from 21 thrombi from 4 mice from each of control and fluvastatin (1-20 μM)–treated groups (E-F). P values calculated by the nonparametric Mann-Whitney test, P > .05 (NS).