VPA induces autophagy and potentiates the effect of CQ in primary t(8;21) AML cells. (A) Primary t(8;21) AML samples were treated or not with VPA (1 mM) for 16 hours, and degradation of long-lived proteins was measured. (B) The AML samples were treated with VPA (1 mM), CQ (12.5 µM), or both for 48 hours. Cell viability was determined by FACS analysis of PI-stained cells and presented as (left) raw data or (right) normalized to the untreated control. **P < .01.