Role of CD40L on the production of hemopoietic cytokines by SCs. (A) The SC expression of IL-6, IL-7, and GM-CSF mRNAs was measured in SCs cultured alone or in the presence of T cells, anti-CD40L Ab, or rWnt10b. (B) Levels of IL-6, IL-7, and GM-SCF in the culture media of SCs from sham-operated and ovx WT mice, TCRβKO mice, and TCRβKO mice previously subjected to adoptive transfer of WT T cells. (C-E) Levels of IL-6, IL-7, and GM-SCF as measured by enzyme-linked immunosorbent assay in the 48-hour culture media of SCs purified from sham operated and ovx WT and CD40L^−/− mice. The first 4 bars to the left show data from WT and CD40L^−/− mice. The last 3 bars to the right show data from WT mice treated with irrelevant Ab or MR-1 Ab. In these experiments, BM was cultured for 1 week, SCs were then purified, and cytokine was measured by enzyme-linked immunosorbent assay in the 48-hour culture media. (F) Effect of ovx on the mRNA expression of Wnt10b in BM T cells. The first 4 bars to the left show WT and CD40L^−/− mice. The last 4 bars to the right show WT mice treated with irrelevant Ab or MR-1 Ab. *P < .05 compared with the corresponding sham-operated group.