Analysis of the effects (mean + SEM) of ovx in WT and Wnt10b^−/− mice. (A) Effects of ovx on the relative frequency of BM LSK cells. (B-C) Effect of ovx on the relative frequency of ST-HSPCs/MPP and LT-HSPCs. (D-F) Effect of ovx on peripheral blood cell expansion after primary competitive repopulation. The percentage of CD11b⁺, GR-1⁺, and B220⁺ cells in the peripheral blood of lethally irradiated WT recipient mice are shown. CD45.2⁺ WT mice and Wnt10b^−/− mice were killed 2 weeks after ovx or sham operation. Their BM was then mixed with BM from intact CD45.1⁺ WT mice at a ratio of 1:2 and injected into lethally irradiated CD45.1⁺ host mice. (G-J) Survival analysis of WT mice transplanted with limiting number of BM cells derived from WT and Wnt10b^−/− mice. (G-H) Donor mice were intact WT or Wnt10b^−/− mice. Recipients were WT mice subjected to sham operation or ovx 2 weeks before transplantation. (I-J) Donor mice were WT mice subjected to sham operation or ovx 2 weeks before transplantation. Recipient mice were intact WT mice. n = 10 in each group. *P < .05 compared with the corresponding sham-operated group.