Individual TFs interact in a complex network to regulate the arteriovenous fresh profile. A schematic overview of the complex interaction by the TFs underlying the regulation of the arteriovenous fresh profile. (A-B) Pie diagrams representing the proportion of arterial (A) or venous (B) genes of the arteriovenous fresh profile regulated by 0 (blue), 1 (purple), 2 (orange), 3 (green), or >3 (beige) TFs. Corresponding absolute numbers are listed in the table on the right of the panel. (C) To summarize all information on the effect of TF overexpression on the arteriovenous fresh profile, we composed an interaction network of the 8 TFs and the arteriovenous fresh profile. Because Aff3 did not regulate any gene of the signature, nor was it regulated by the other TFs, it is not included in the network. Each TF hub (represented by rounded boxes) is drawn in a different color and interactions originating from each hub are shown by arrows (induction) or vertical lines (inhibition) in the corresponding color. Genes are assigned to the hubs according to the TF by which they were most strongly regulated. For those genes exclusively regulated by 1 TF, the box of that gene is drawn in dark color, whereas for genes that are regulated by >1 TF, the box is drawn in light color. TFs are in oval boxes, arterial genes are in rectangular boxes with full lines, and venous genes are in rectangular boxes with dashed lines.