Kinetics of clone appearance, size, persistence, and lineage content. (A) Lines depict changes throughout time in the total size of each barcoded (GFP⁺) clone in primary mice, extrapolated from the BM sample and distinguishing those only seen at the time indicated in the panel (shown as red lines) vs those also detected at other times (shown as black lines). Gray shaded region denotes the limit of detection by MPS. Each pie chart shows the relative distributions of different types of clones present at different times (of the line plot, above) according to the diversity of lineages present at that time. (B) Different patterns of lineage content of clones present at 27 weeks posttransplant. 13 clonal patterns were defined based on the changes (absolute increases or decreases) detected in the lineage content of each clone through time as illustrated by the representative plots shown. Colored dotted lines denote the absence of specific cell lineages at the indicated detection limit.