Figure 7
Figure 7. Transient inhibition of 11βHSD1 enhances capacity of CD8α+ to cross-present antigen. Wild-type female B6 mice were treated with one or more of anti-DEC205:OVA (200 µL supernatant), CBX (2 mg), or CpG 1826 (100 µg) given by intraperitoneal injection before evaluating the endogenous anti-OVA CD8 T-cell response 7 days later. (A) Representative flow cytometric plots showing intracellular IFN-γ staining on gated CD8 T cells following restimulation overnight with relevant (SIINFEKL) or irrelevant peptide. Gates set according to irrelevant peptide. (B) Summary data for by Kb:SIINFEKL pentamer staining (left) in spleens or specific intracellular IFN-γ generation (right) are shown.

Transient inhibition of 11βHSD1 enhances capacity of CD8α+to cross-present antigen. Wild-type female B6 mice were treated with one or more of anti-DEC205:OVA (200 µL supernatant), CBX (2 mg), or CpG 1826 (100 µg) given by intraperitoneal injection before evaluating the endogenous anti-OVA CD8 T-cell response 7 days later. (A) Representative flow cytometric plots showing intracellular IFN-γ staining on gated CD8 T cells following restimulation overnight with relevant (SIINFEKL) or irrelevant peptide. Gates set according to irrelevant peptide. (B) Summary data for by Kb:SIINFEKL pentamer staining (left) in spleens or specific intracellular IFN-γ generation (right) are shown.

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