C/EBPα-p42 regulates miR-30c during granulopoiesis. (A) Lentiviral overexpression of C/EBPα in K562 cells. Total RNA was isolated at day 7 and analyzed by Q-RT-PCR with oligos for miR-30c and miR-223. Values were normalized to U6. Data are represented as mean ± SD from 3 independent experiments. *P ≤ .05. (B) K562-C/EBPα-p42ER, (C) K562-C/EBPα-p30ER, and (D) K562-ER cells were induced with β-estradiol (5 µM) for respective time points. Total RNA was analyzed by Q-RT-PCR with oligos for miR-30c. Values were normalized to U6. Data are represented as mean ± SD from 3 independent experiments. *P ≤ .05. (E) U937 cells were induced with retinoic acid (1 µM) for respective time points. Total RNA was analyzed by Q-RT-PCR with oligos for miR-30c. Values were normalized to U6. Data are represented as mean ± SD from 3 independent experiments. *P ≤ .05. (F) Hematopoietic CD34⁺ cells were cultured as discussed in Methods. Total RNA was isolated at different time points and analyzed by Q-RT-PCR with oligos for miR-30c. Values were normalized to U6. Data are represented as mean ± SD from 2 independent experiments.