miR-30c is necessary for C/EBPα to block Notch1 and to induce myeloid differentiation. (A) K562 cells were cotransfected with pcDNA3-C/EBPα or pcDNA3-empty vector along with LNA oligonucleotides for miR-30c or scramble (control) for 48 hours. Total protein was analyzed by western blot analysis with anti-Notch1 antibody. Values below the gel image indicate the Notch1 protein levels normalized to GAPDH. (B) FACS analysis of CD11b expression during blocking of miR-30c with LNA oligonucleotides in β-estradiol (5 µM)–treated K562-C/EBPα-p42ER cells by flow cytometry. Representative FACS data are from K562-C/EBPα-p42ER cells transfected for 24 hours. Total data are represented as mean ± SD from 2 representative experiments. *P ≤ .005.