American Society of Hematology

Figure 6

$Figure 6. Transient translocation of myosin to DRM raft fraction. (A) Time-dependent translocation of myosin from nonraft fraction (lanes 1-6) to DRM raft fraction (lanes 7-12) in platelets by thrombin stimulation for 0 seconds (lanes 1 and 7), 30 seconds (lanes 2 and 8), 1 minute (lanes 3 and 9), 5 minutes (lanes 4 and 10), 15 minutes (lanes 5 and 11), and 60 minutes (lanes 6 and 12). Immunoblotting was performed with a myosin heavy-chain antibody. (B) ATPase-activity-dependent translocation of myosin to DRM raft fraction. Immunoblotting with antimyosin antibody of DRM raft fraction (lane R) and nonraft fraction (lane N) in resting platelets (left panel) and thrombin-stimulated platelets in the absence (middle panel) or presence (right panel) of 100 μM myosin ATPase inhibitor blebbistatin. (C) PAR-dependent translocation of myosin to DRM raft fraction. Immunoblotting with antimyosin antibody of DRM raft fraction (lanes 1-4) and nonraft fraction (lanes 5-8) in resting platelets (lanes 1 and 5) and platelets stimulated with 0.2 U/mL thrombin (lanes 2 and 6), 25 μM TRAP (lanes 3 and 7), and 20 μM adenosine 5′-diphosphate (lanes 4 and 8) for 3 minutes.$

Transient translocation of myosin to DRM raft fraction. (A) Time-dependent translocation of myosin from nonraft fraction (lanes 1-6) to DRM raft fraction (lanes 7-12) in platelets by thrombin stimulation for 0 seconds (lanes 1 and 7), 30 seconds (lanes 2 and 8), 1 minute (lanes 3 and 9), 5 minutes (lanes 4 and 10), 15 minutes (lanes 5 and 11), and 60 minutes (lanes 6 and 12). Immunoblotting was performed with a myosin heavy-chain antibody. (B) ATPase-activity-dependent translocation of myosin to DRM raft fraction. Immunoblotting with antimyosin antibody of DRM raft fraction (lane R) and nonraft fraction (lane N) in resting platelets (left panel) and thrombin-stimulated platelets in the absence (middle panel) or presence (right panel) of 100 μM myosin ATPase inhibitor blebbistatin. (C) PAR-dependent translocation of myosin to DRM raft fraction. Immunoblotting with antimyosin antibody of DRM raft fraction (lanes 1-4) and nonraft fraction (lanes 5-8) in resting platelets (lanes 1 and 5) and platelets stimulated with 0.2 U/mL thrombin (lanes 2 and 6), 25 μM TRAP (lanes 3 and 7), and 20 μM adenosine 5′-diphosphate (lanes 4 and 8) for 3 minutes.

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