Transgenic Bcl-2 rescues programmed cell death but fails to restore T-cell development in DN-IkTg mice. (A) Thymocyte profiles of BCL2.DN-IkTg mice with progressing age. Contour plots show CD4/CD8 profiles of BCL2.DN-IkTg and control DN-IkTg thymocytes at the indicated age. (B) Thymocyte profiles of BCL2Tg and BCL2.DN-IkTg mice. Contour plots show CD4 vs CD8 profiles of total thymocytes. Contour plots are from age-matched mice (81 days old) and are representative of 4 independent experiments with 4 BCL2Tg and 7 BCL2.DN-IkTg mice. (C) Survival kinetics of BCL2.DN-IkTg CD4SP thymocytes in vitro. Cell viabilities were determined by propidium iodide exclusion at the indicated time points. Data show the summary of 2 independent experiments with 2 BCL2Tg and 3 BCL2.DN-IkTg mice. (D) Total thymocyte numbers of BCL2Tg and BCL2.DN-IkTg mice. Bar graphs show the mean ± SEM of at least 4 independent experiments with 4 BCL2Tg and 7 BCL2Tg.DN-IkTg mice. (E) Intracellular Ki-67 staining in HSA^hi and HSA^loTCRβ⁺ CD4SP thymocytes. Data are representative of 4 independent experiments.