Clonal expansion of postselection CD4⁺CD8^lo intermediate cells in DN-IkTg thymocytes. (A) Thymocyte profiles of DN-IkTg and MHCIIKO.DN-IkTg mice. Contour plots show CD4/CD8 profiles of total and TCRβ^hi-gated thymocytes. Data are representative of 5 independent experiments with 6 DN-IkTg and 5 MHCIIKO.DN-IkTg mice. (B) Thymocyte differentiation in MHCIIKO.DN-IkTg mice. HSA vs TCRβ profiles of WT, DN-IkTg, MHCIIKO, and MHCIIKO.DN-IkTg mice. Data are representative of 4 independent experiments with 4 WT, 6 DN-IkTg, 5 MHCIIKO, and 5 MHCIIKO.DN-IkTg mice. (C) Total thymocyte numbers of DN-IkTg and MHCIIKO.DN-IkTg mice. The bar graph shows the mean ± SEM from 3 independent experiments with 10 DN-IkTg and 5 MHCIIKO.DN-IkTg mice. (D) TCRβ^hi CD4SP thymocyte numbers of age-matched DN-IkTg and MHCIIKO.DN-IkTg mice. The bar graph shows the mean ± SEM from 3 independent experiments with 10 DN-IkTg and 5 MHCIIKO.DN-IkTg mice. (E) Thymocyte profiles and cell numbers of HY.RAG.DN-IkTg mice. CD4/CD8 profiles are representative of 5 independent experiments. The bar graph shows the mean ± SEM from 5 independent experiments with 6 HY.RAG female and 9 HY.RAG.DN-IkTg female mice.