Expression of plasma miRNA signature for aGVHD diagnosis in a training set. (A) Expression of miR-423, miR-199a-3p, miR-93*, miR-377, miR-155, and miR-30a in plasma of aGVHD (n = 59) and non-GVHD patients (n = 19) 6 weeks after HCT. P values were calculated using the Mann-Whitney test. (B) ROC analysis for individual miRNAs and combined miRNA panel. Logistic regression demonstrated that a linear combination of values for miR-423, miR-199a-3p, miR-93*, and miR-377 produced the best model for aGVHD diagnosis; the equation was “Combined miRNA panel = 5.514 + 0.255 × miR-423 − 0.427 × miR-199a-3p + 0.522 × miR-93* − 1.161 × miR-377.” ROC curve of miRNA panel was generated based on the predicted probability (P) for each patient. Predicted probability = Exp (combined miRNA panel)/ [1+Exp (combined miRNA panel)]. (C) Comparison of sIL-2Rα protein levels in plasma between aGVHD and non-GVHD patients 6 weeks after HCT. P values were calculated using the Mann-Whitney test.